Using the appropriate power cord supplied with the blotter,connect the blotter control unit to an outlet.Switch on the Power Button located on the control units rear panel.For each gel, use four sheets of 0.83mm thick Western blottingfilter paper and one sheet of nitrocellulose or PVDF membrane cutto the same size.Equilibrate filter paper and membrane in Thermo Scientific Pierce1-Step Transfer Buffer for at least 5 minutes. Use sufficient bufferto cover filter paper and membrane 50mL per mini-sized 7cmx 8.4cm sandwich and 100mL per midi-sized 8cm x 13.5cmsandwich.Note PVDF membrane must be wetted with methanol orethanol before equilibration in Pierce 1-Step Transfer Buffer.After electrophoresis, remove gels from cassettes and brieflyplace into tray containing deionized water or transfer buffer. This willensure even wetting, facilitate proper gel placement and improvegel contact with the membrane.Assemble and center sandwiches on bottom of cassette anodeto ensure even pressure.
Other Specifications Download Specification | |
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Types Of System | Horizontal Gel Electrophoresis system |
Applications | To separate charged molecules like DNA/RNA and proteins according to the size |
Series Of Electrophoresis | Small |
No. Of Maximum Samples Which Can Run | 4 Nos |
Body Material Of Electrophoresis Unit | metallic powder coated |
Size Of Unit (WxLxH) | 25727835 mm |
Gel Tray Requirements | The tray should be UV proof and should have integrated fluorescent ruler which should get illuminated on exposure to UV lights for easy and safe calculation of the band movement |
Material Of Gel Tray | Acrylic |
Width Of The Gel Tray | 0 cm |
Length Of The Gel Tray | 0 cm |
Thickness Of Combs And Colour Code | 1mm-white,1.5mm- Red,2mm-Blue |
All Combs Of Adjustable Height | Yes |
No. Of Wellcombs | 0 Nos |
Width Of Well,cm | 0 cm |
Length Of Well,cm | 0 cm |
Volume Of Gel,ml | NA ml |
Type Of Electrode | Platinum |
The Base Buffer Volume,ml | 100 ml |
Details Of Other Components Included | yes |
Required Dye Chemicals | 0 |
Specified Voltage For Dye Migration | 0 V |
Dye Migration Rate | 0 cm/hr |
Maxi.output Voltage Of Power Supply Unit | 25 V |
Output Current | 2 mA |
Ouput Wattage,W | 25 W |
Constant Voltage | Yes |
Constant Current | Yes |
Display | LCD display |
Control | Microprocessor controlled |
No. Of Electrode Terminal Ports | 2 Nos |
Alarm For Time Completion | Yes |
Timer Range Up To | 60 minutes |
Autostart After Power Breakdown | Yes |
Operating Voltage | 230 V |
Operating Temperature Range | 1 deg.C |
Power Unit Dimension (LxWxH) | 6441088606 mm |
Weight Of Power Unit | 20 kg |
Warranty Period Of The System | 1 years |
Details Of Consumables To Be Supplied | NA |
Installation,commissioning And Training Part Of The Supply | Yes |